cell culture reagents dulbeccos modified eagle medium (dmem) Search Results


dulbecco’s modified eagle’s medium supplemented with 2% horse serum and antibiotics (differentiation medium)  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures dulbecco’s modified eagle’s medium supplemented with 2% horse serum and antibiotics (differentiation medium)
Dulbecco’s Modified Eagle’s Medium Supplemented With 2% Horse Serum And Antibiotics (Differentiation Medium), supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dulbecco’s modified eagle’s medium supplemented with 2% horse serum and antibiotics (differentiation medium) - by Bioz Stars, 2026-10
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bv-2 microglia cell culture dulbecco’s modified eagle’s medium (dmem) and fetal bovine serum (fbs)  (Welgene inc)
90
Welgene inc bv-2 microglia cell culture dulbecco’s modified eagle’s medium (dmem) and fetal bovine serum (fbs)
Bv 2 Microglia Cell Culture Dulbecco’s Modified Eagle’s Medium (Dmem) And Fetal Bovine Serum (Fbs), supplied by Welgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
bv-2 microglia cell culture dulbecco’s modified eagle’s medium (dmem) and fetal bovine serum (fbs) - by Bioz Stars, 2026-10
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cell culture medium dulbecco’s modified eagle’s medium (dmem) provided by  (MatTek)
90
MatTek cell culture medium dulbecco’s modified eagle’s medium (dmem) provided by
Cell Culture Medium Dulbecco’s Modified Eagle’s Medium (Dmem) Provided By, supplied by MatTek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+reagents+dulbeccos+modified+eagle+medium+(dmem)/cell+culture+medium+dulbecco%E2%80%99s+modified+eagle%E2%80%99s+medium++dmem++provided+by/pm27439971-68-19-29
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cell culture medium dulbecco’s modified eagle’s medium (dmem) provided by - by Bioz Stars, 2026-10
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culture plates with dmem (dulbecco’s modified eagle medium)  (Corning Life Sciences)
90
Corning Life Sciences culture plates with dmem (dulbecco’s modified eagle medium)
Culture Plates With Dmem (Dulbecco’s Modified Eagle Medium), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+reagents+dulbeccos+modified+eagle+medium+(dmem)/culture+plates+with+dmem++dulbecco%E2%80%99s+modified+eagle+medium+/bio_rxiv__2022__03__30__486486-314-11-19
Average 90 stars, based on 1 article reviews
culture plates with dmem (dulbecco’s modified eagle medium) - by Bioz Stars, 2026-10
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rpmi 1640 + glutamine supplemented with 10% fbs  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures rpmi 1640 + glutamine supplemented with 10% fbs
Differentiation of HL60 model system using two different culturing conditions. (A) Schema of HL60 myeloid differentiation model into monocytes and granulocytes using vitamin D3 (Vit D3) and all-trans retinoic acid (ATRA) respectively. (B) Bar plot shows mean fluorescence intensity (MFI) of CD14-APC-H7 and CD11b-FITC at 6, 12, 24, 48 and 72 h of induction time points with Vit D3 and ATRA (** = 0.01, *** = 0.001, **** = 0.0001). An unpaired t -test was performed taking a 6 h data point as a reference using GraphPad Prism version 8.2.1. (C) Contour plots showing gating of stained cells with IgG-APC-H7, followed by staining uninduced cells, and Vit D3 induced HL60 cells (72 h induction) with CD14-APC H7 (from left to right contour plots). The X -axis and Y -axis of the contour plots show CD14-APC H7 and forward scatter (A = area) respectively. (D) Bar plots representing MFI of the CD14-APC H7 intensity at 72 h post-Vit D3 induction. The MFI of CD14-APC H7 positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). An unpaired t -test was performed using GraphPad Prism version 8.2.1. (E) Similar to panel C, HL60 cells were induced with ATRA for 72 h and IgG-FITC and CD11b-FITC were used in this experiment. The X -axis and Y -axis of the contour plots show CD11b-FITC and forward scatter (A = area) respectively. (F) Bar plots representing MFI of the CD11b-FITC intensity at 72 h post-ATRA induction. The MFI of CD11b-FITC positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). A statistical test was performed as mentioned in panel D (G) Bar graphs represent the percentage of CD14 positive cells and MFI of CD14-APC H7 positive HL60 cells at 0 and 72 h in RPMI with 10% <t>FBS</t> <t>and</t> <t>IMDM</t> with 20% FBS. The induced cells were compared with their respective uninduced cells in both the culturing conditions in the HL60 differentiation in the IMDM and RPMI media conditions (** = 0.01, *** = 0.001). A statistical test was performed as mentioned in panel (D) (H) Similar to panel G but induction was carried out with ATRA for 72 h. (I) May Grünwald Giemsa staining showing nuclear morphology changes upon HL60 differentiation into monocytes and granulocytes in IMDM (upper panel) and RPMI (lower panel) culturing and induction conditions. The image is taken in Olympus IX83 inverted microscope using CellSens imaging software and 40× magnification.
Rpmi 1640 + Glutamine Supplemented With 10% Fbs, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+reagents+dulbeccos+modified+eagle+medium+(dmem)/dulbecco%E2%80%99s+modified+eagle%E2%80%99s+medium+supplemented+with+10++fetal+bovine+serum+and+antibiotics++growth+medium+/pmc09932920-210-41-24
Average 90 stars, based on 1 article reviews
rpmi 1640 + glutamine supplemented with 10% fbs - by Bioz Stars, 2026-10
90/100 stars
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cell culture media dulbecco’s modified eagle’s medium (dmem), -glutamine, penicillin and streptomycin  (Biochrom)
90
Biochrom cell culture media dulbecco’s modified eagle’s medium (dmem), -glutamine, penicillin and streptomycin
Differentiation of HL60 model system using two different culturing conditions. (A) Schema of HL60 myeloid differentiation model into monocytes and granulocytes using vitamin D3 (Vit D3) and all-trans retinoic acid (ATRA) respectively. (B) Bar plot shows mean fluorescence intensity (MFI) of CD14-APC-H7 and CD11b-FITC at 6, 12, 24, 48 and 72 h of induction time points with Vit D3 and ATRA (** = 0.01, *** = 0.001, **** = 0.0001). An unpaired t -test was performed taking a 6 h data point as a reference using GraphPad Prism version 8.2.1. (C) Contour plots showing gating of stained cells with IgG-APC-H7, followed by staining uninduced cells, and Vit D3 induced HL60 cells (72 h induction) with CD14-APC H7 (from left to right contour plots). The X -axis and Y -axis of the contour plots show CD14-APC H7 and forward scatter (A = area) respectively. (D) Bar plots representing MFI of the CD14-APC H7 intensity at 72 h post-Vit D3 induction. The MFI of CD14-APC H7 positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). An unpaired t -test was performed using GraphPad Prism version 8.2.1. (E) Similar to panel C, HL60 cells were induced with ATRA for 72 h and IgG-FITC and CD11b-FITC were used in this experiment. The X -axis and Y -axis of the contour plots show CD11b-FITC and forward scatter (A = area) respectively. (F) Bar plots representing MFI of the CD11b-FITC intensity at 72 h post-ATRA induction. The MFI of CD11b-FITC positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). A statistical test was performed as mentioned in panel D (G) Bar graphs represent the percentage of CD14 positive cells and MFI of CD14-APC H7 positive HL60 cells at 0 and 72 h in RPMI with 10% <t>FBS</t> <t>and</t> <t>IMDM</t> with 20% FBS. The induced cells were compared with their respective uninduced cells in both the culturing conditions in the HL60 differentiation in the IMDM and RPMI media conditions (** = 0.01, *** = 0.001). A statistical test was performed as mentioned in panel (D) (H) Similar to panel G but induction was carried out with ATRA for 72 h. (I) May Grünwald Giemsa staining showing nuclear morphology changes upon HL60 differentiation into monocytes and granulocytes in IMDM (upper panel) and RPMI (lower panel) culturing and induction conditions. The image is taken in Olympus IX83 inverted microscope using CellSens imaging software and 40× magnification.
Cell Culture Media Dulbecco’s Modified Eagle’s Medium (Dmem), Glutamine, Penicillin And Streptomycin, supplied by Biochrom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+reagents+dulbeccos+modified+eagle+medium+(dmem)/cell+culture+media+dulbecco%E2%80%99s+modified+eagle%E2%80%99s+medium++dmem++++glutamine++penicillin+and+streptomycin/10__1042_slash_bj3301107-39-13-14
Average 90 stars, based on 1 article reviews
cell culture media dulbecco’s modified eagle’s medium (dmem), -glutamine, penicillin and streptomycin - by Bioz Stars, 2026-10
90/100 stars
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dulbecco’s modified eagle’s medium  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures dulbecco’s modified eagle’s medium
Differentiation of HL60 model system using two different culturing conditions. (A) Schema of HL60 myeloid differentiation model into monocytes and granulocytes using vitamin D3 (Vit D3) and all-trans retinoic acid (ATRA) respectively. (B) Bar plot shows mean fluorescence intensity (MFI) of CD14-APC-H7 and CD11b-FITC at 6, 12, 24, 48 and 72 h of induction time points with Vit D3 and ATRA (** = 0.01, *** = 0.001, **** = 0.0001). An unpaired t -test was performed taking a 6 h data point as a reference using GraphPad Prism version 8.2.1. (C) Contour plots showing gating of stained cells with IgG-APC-H7, followed by staining uninduced cells, and Vit D3 induced HL60 cells (72 h induction) with CD14-APC H7 (from left to right contour plots). The X -axis and Y -axis of the contour plots show CD14-APC H7 and forward scatter (A = area) respectively. (D) Bar plots representing MFI of the CD14-APC H7 intensity at 72 h post-Vit D3 induction. The MFI of CD14-APC H7 positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). An unpaired t -test was performed using GraphPad Prism version 8.2.1. (E) Similar to panel C, HL60 cells were induced with ATRA for 72 h and IgG-FITC and CD11b-FITC were used in this experiment. The X -axis and Y -axis of the contour plots show CD11b-FITC and forward scatter (A = area) respectively. (F) Bar plots representing MFI of the CD11b-FITC intensity at 72 h post-ATRA induction. The MFI of CD11b-FITC positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). A statistical test was performed as mentioned in panel D (G) Bar graphs represent the percentage of CD14 positive cells and MFI of CD14-APC H7 positive HL60 cells at 0 and 72 h in RPMI with 10% <t>FBS</t> <t>and</t> <t>IMDM</t> with 20% FBS. The induced cells were compared with their respective uninduced cells in both the culturing conditions in the HL60 differentiation in the IMDM and RPMI media conditions (** = 0.01, *** = 0.001). A statistical test was performed as mentioned in panel (D) (H) Similar to panel G but induction was carried out with ATRA for 72 h. (I) May Grünwald Giemsa staining showing nuclear morphology changes upon HL60 differentiation into monocytes and granulocytes in IMDM (upper panel) and RPMI (lower panel) culturing and induction conditions. The image is taken in Olympus IX83 inverted microscope using CellSens imaging software and 40× magnification.
Dulbecco’s Modified Eagle’s Medium, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+reagents+dulbeccos+modified+eagle+medium+(dmem)/dulbecco%E2%80%99s+modified+eagle+medium/pm29580479-17-38-44
Average 90 stars, based on 1 article reviews
dulbecco’s modified eagle’s medium - by Bioz Stars, 2026-10
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higluta xltm dulbecco’s modified eagle’s medium (high glucose) cell culture medium  (HiMedia Laboratories)
90
HiMedia Laboratories higluta xltm dulbecco’s modified eagle’s medium (high glucose) cell culture medium
Differentiation of HL60 model system using two different culturing conditions. (A) Schema of HL60 myeloid differentiation model into monocytes and granulocytes using vitamin D3 (Vit D3) and all-trans retinoic acid (ATRA) respectively. (B) Bar plot shows mean fluorescence intensity (MFI) of CD14-APC-H7 and CD11b-FITC at 6, 12, 24, 48 and 72 h of induction time points with Vit D3 and ATRA (** = 0.01, *** = 0.001, **** = 0.0001). An unpaired t -test was performed taking a 6 h data point as a reference using GraphPad Prism version 8.2.1. (C) Contour plots showing gating of stained cells with IgG-APC-H7, followed by staining uninduced cells, and Vit D3 induced HL60 cells (72 h induction) with CD14-APC H7 (from left to right contour plots). The X -axis and Y -axis of the contour plots show CD14-APC H7 and forward scatter (A = area) respectively. (D) Bar plots representing MFI of the CD14-APC H7 intensity at 72 h post-Vit D3 induction. The MFI of CD14-APC H7 positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). An unpaired t -test was performed using GraphPad Prism version 8.2.1. (E) Similar to panel C, HL60 cells were induced with ATRA for 72 h and IgG-FITC and CD11b-FITC were used in this experiment. The X -axis and Y -axis of the contour plots show CD11b-FITC and forward scatter (A = area) respectively. (F) Bar plots representing MFI of the CD11b-FITC intensity at 72 h post-ATRA induction. The MFI of CD11b-FITC positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). A statistical test was performed as mentioned in panel D (G) Bar graphs represent the percentage of CD14 positive cells and MFI of CD14-APC H7 positive HL60 cells at 0 and 72 h in RPMI with 10% <t>FBS</t> <t>and</t> <t>IMDM</t> with 20% FBS. The induced cells were compared with their respective uninduced cells in both the culturing conditions in the HL60 differentiation in the IMDM and RPMI media conditions (** = 0.01, *** = 0.001). A statistical test was performed as mentioned in panel (D) (H) Similar to panel G but induction was carried out with ATRA for 72 h. (I) May Grünwald Giemsa staining showing nuclear morphology changes upon HL60 differentiation into monocytes and granulocytes in IMDM (upper panel) and RPMI (lower panel) culturing and induction conditions. The image is taken in Olympus IX83 inverted microscope using CellSens imaging software and 40× magnification.
Higluta Xltm Dulbecco’s Modified Eagle’s Medium (High Glucose) Cell Culture Medium, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
higluta xltm dulbecco’s modified eagle’s medium (high glucose) cell culture medium - by Bioz Stars, 2026-10
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dulbecco’s modified eagle medium (dmem  (CELLnTEC Advanced Cell Systems AG)
90
CELLnTEC Advanced Cell Systems AG dulbecco’s modified eagle medium (dmem
Differentiation of HL60 model system using two different culturing conditions. (A) Schema of HL60 myeloid differentiation model into monocytes and granulocytes using vitamin D3 (Vit D3) and all-trans retinoic acid (ATRA) respectively. (B) Bar plot shows mean fluorescence intensity (MFI) of CD14-APC-H7 and CD11b-FITC at 6, 12, 24, 48 and 72 h of induction time points with Vit D3 and ATRA (** = 0.01, *** = 0.001, **** = 0.0001). An unpaired t -test was performed taking a 6 h data point as a reference using GraphPad Prism version 8.2.1. (C) Contour plots showing gating of stained cells with IgG-APC-H7, followed by staining uninduced cells, and Vit D3 induced HL60 cells (72 h induction) with CD14-APC H7 (from left to right contour plots). The X -axis and Y -axis of the contour plots show CD14-APC H7 and forward scatter (A = area) respectively. (D) Bar plots representing MFI of the CD14-APC H7 intensity at 72 h post-Vit D3 induction. The MFI of CD14-APC H7 positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). An unpaired t -test was performed using GraphPad Prism version 8.2.1. (E) Similar to panel C, HL60 cells were induced with ATRA for 72 h and IgG-FITC and CD11b-FITC were used in this experiment. The X -axis and Y -axis of the contour plots show CD11b-FITC and forward scatter (A = area) respectively. (F) Bar plots representing MFI of the CD11b-FITC intensity at 72 h post-ATRA induction. The MFI of CD11b-FITC positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). A statistical test was performed as mentioned in panel D (G) Bar graphs represent the percentage of CD14 positive cells and MFI of CD14-APC H7 positive HL60 cells at 0 and 72 h in RPMI with 10% <t>FBS</t> <t>and</t> <t>IMDM</t> with 20% FBS. The induced cells were compared with their respective uninduced cells in both the culturing conditions in the HL60 differentiation in the IMDM and RPMI media conditions (** = 0.01, *** = 0.001). A statistical test was performed as mentioned in panel (D) (H) Similar to panel G but induction was carried out with ATRA for 72 h. (I) May Grünwald Giemsa staining showing nuclear morphology changes upon HL60 differentiation into monocytes and granulocytes in IMDM (upper panel) and RPMI (lower panel) culturing and induction conditions. The image is taken in Olympus IX83 inverted microscope using CellSens imaging software and 40× magnification.
Dulbecco’s Modified Eagle Medium (Dmem, supplied by CELLnTEC Advanced Cell Systems AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+reagents+dulbeccos+modified+eagle+medium+(dmem)/dulbecco%E2%80%99s+modified+eagle+medium++dmem/10__1002_slash_adfm__201904007-229-22-2
Average 90 stars, based on 1 article reviews
dulbecco’s modified eagle medium (dmem - by Bioz Stars, 2026-10
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osteogenic cell culture medium modified eagle's medium dulbecco  (Biochrom)
90
Biochrom osteogenic cell culture medium modified eagle's medium dulbecco
Differentiation of HL60 model system using two different culturing conditions. (A) Schema of HL60 myeloid differentiation model into monocytes and granulocytes using vitamin D3 (Vit D3) and all-trans retinoic acid (ATRA) respectively. (B) Bar plot shows mean fluorescence intensity (MFI) of CD14-APC-H7 and CD11b-FITC at 6, 12, 24, 48 and 72 h of induction time points with Vit D3 and ATRA (** = 0.01, *** = 0.001, **** = 0.0001). An unpaired t -test was performed taking a 6 h data point as a reference using GraphPad Prism version 8.2.1. (C) Contour plots showing gating of stained cells with IgG-APC-H7, followed by staining uninduced cells, and Vit D3 induced HL60 cells (72 h induction) with CD14-APC H7 (from left to right contour plots). The X -axis and Y -axis of the contour plots show CD14-APC H7 and forward scatter (A = area) respectively. (D) Bar plots representing MFI of the CD14-APC H7 intensity at 72 h post-Vit D3 induction. The MFI of CD14-APC H7 positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). An unpaired t -test was performed using GraphPad Prism version 8.2.1. (E) Similar to panel C, HL60 cells were induced with ATRA for 72 h and IgG-FITC and CD11b-FITC were used in this experiment. The X -axis and Y -axis of the contour plots show CD11b-FITC and forward scatter (A = area) respectively. (F) Bar plots representing MFI of the CD11b-FITC intensity at 72 h post-ATRA induction. The MFI of CD11b-FITC positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). A statistical test was performed as mentioned in panel D (G) Bar graphs represent the percentage of CD14 positive cells and MFI of CD14-APC H7 positive HL60 cells at 0 and 72 h in RPMI with 10% <t>FBS</t> <t>and</t> <t>IMDM</t> with 20% FBS. The induced cells were compared with their respective uninduced cells in both the culturing conditions in the HL60 differentiation in the IMDM and RPMI media conditions (** = 0.01, *** = 0.001). A statistical test was performed as mentioned in panel (D) (H) Similar to panel G but induction was carried out with ATRA for 72 h. (I) May Grünwald Giemsa staining showing nuclear morphology changes upon HL60 differentiation into monocytes and granulocytes in IMDM (upper panel) and RPMI (lower panel) culturing and induction conditions. The image is taken in Olympus IX83 inverted microscope using CellSens imaging software and 40× magnification.
Osteogenic Cell Culture Medium Modified Eagle's Medium Dulbecco, supplied by Biochrom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
osteogenic cell culture medium modified eagle's medium dulbecco - by Bioz Stars, 2026-10
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cell complete medium containing 4.5 g/l glucose dulbecco’s modified eagle medium (dmem)  (Corning Life Sciences)
90
Corning Life Sciences cell complete medium containing 4.5 g/l glucose dulbecco’s modified eagle medium (dmem)
Differentiation of HL60 model system using two different culturing conditions. (A) Schema of HL60 myeloid differentiation model into monocytes and granulocytes using vitamin D3 (Vit D3) and all-trans retinoic acid (ATRA) respectively. (B) Bar plot shows mean fluorescence intensity (MFI) of CD14-APC-H7 and CD11b-FITC at 6, 12, 24, 48 and 72 h of induction time points with Vit D3 and ATRA (** = 0.01, *** = 0.001, **** = 0.0001). An unpaired t -test was performed taking a 6 h data point as a reference using GraphPad Prism version 8.2.1. (C) Contour plots showing gating of stained cells with IgG-APC-H7, followed by staining uninduced cells, and Vit D3 induced HL60 cells (72 h induction) with CD14-APC H7 (from left to right contour plots). The X -axis and Y -axis of the contour plots show CD14-APC H7 and forward scatter (A = area) respectively. (D) Bar plots representing MFI of the CD14-APC H7 intensity at 72 h post-Vit D3 induction. The MFI of CD14-APC H7 positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). An unpaired t -test was performed using GraphPad Prism version 8.2.1. (E) Similar to panel C, HL60 cells were induced with ATRA for 72 h and IgG-FITC and CD11b-FITC were used in this experiment. The X -axis and Y -axis of the contour plots show CD11b-FITC and forward scatter (A = area) respectively. (F) Bar plots representing MFI of the CD11b-FITC intensity at 72 h post-ATRA induction. The MFI of CD11b-FITC positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). A statistical test was performed as mentioned in panel D (G) Bar graphs represent the percentage of CD14 positive cells and MFI of CD14-APC H7 positive HL60 cells at 0 and 72 h in RPMI with 10% <t>FBS</t> <t>and</t> <t>IMDM</t> with 20% FBS. The induced cells were compared with their respective uninduced cells in both the culturing conditions in the HL60 differentiation in the IMDM and RPMI media conditions (** = 0.01, *** = 0.001). A statistical test was performed as mentioned in panel (D) (H) Similar to panel G but induction was carried out with ATRA for 72 h. (I) May Grünwald Giemsa staining showing nuclear morphology changes upon HL60 differentiation into monocytes and granulocytes in IMDM (upper panel) and RPMI (lower panel) culturing and induction conditions. The image is taken in Olympus IX83 inverted microscope using CellSens imaging software and 40× magnification.
Cell Complete Medium Containing 4.5 G/L Glucose Dulbecco’s Modified Eagle Medium (Dmem), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cell complete medium containing 4.5 g/l glucose dulbecco’s modified eagle medium (dmem) - by Bioz Stars, 2026-10
90/100 stars
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dulbecco’s modified eagle’s medium (dmem) media  (PRIMACYT Cell Culture Technology GmbH)
90
PRIMACYT Cell Culture Technology GmbH dulbecco’s modified eagle’s medium (dmem) media
Differentiation of HL60 model system using two different culturing conditions. (A) Schema of HL60 myeloid differentiation model into monocytes and granulocytes using vitamin D3 (Vit D3) and all-trans retinoic acid (ATRA) respectively. (B) Bar plot shows mean fluorescence intensity (MFI) of CD14-APC-H7 and CD11b-FITC at 6, 12, 24, 48 and 72 h of induction time points with Vit D3 and ATRA (** = 0.01, *** = 0.001, **** = 0.0001). An unpaired t -test was performed taking a 6 h data point as a reference using GraphPad Prism version 8.2.1. (C) Contour plots showing gating of stained cells with IgG-APC-H7, followed by staining uninduced cells, and Vit D3 induced HL60 cells (72 h induction) with CD14-APC H7 (from left to right contour plots). The X -axis and Y -axis of the contour plots show CD14-APC H7 and forward scatter (A = area) respectively. (D) Bar plots representing MFI of the CD14-APC H7 intensity at 72 h post-Vit D3 induction. The MFI of CD14-APC H7 positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). An unpaired t -test was performed using GraphPad Prism version 8.2.1. (E) Similar to panel C, HL60 cells were induced with ATRA for 72 h and IgG-FITC and CD11b-FITC were used in this experiment. The X -axis and Y -axis of the contour plots show CD11b-FITC and forward scatter (A = area) respectively. (F) Bar plots representing MFI of the CD11b-FITC intensity at 72 h post-ATRA induction. The MFI of CD11b-FITC positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). A statistical test was performed as mentioned in panel D (G) Bar graphs represent the percentage of CD14 positive cells and MFI of CD14-APC H7 positive HL60 cells at 0 and 72 h in RPMI with 10% <t>FBS</t> <t>and</t> <t>IMDM</t> with 20% FBS. The induced cells were compared with their respective uninduced cells in both the culturing conditions in the HL60 differentiation in the IMDM and RPMI media conditions (** = 0.01, *** = 0.001). A statistical test was performed as mentioned in panel (D) (H) Similar to panel G but induction was carried out with ATRA for 72 h. (I) May Grünwald Giemsa staining showing nuclear morphology changes upon HL60 differentiation into monocytes and granulocytes in IMDM (upper panel) and RPMI (lower panel) culturing and induction conditions. The image is taken in Olympus IX83 inverted microscope using CellSens imaging software and 40× magnification.
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Differentiation of HL60 model system using two different culturing conditions. (A) Schema of HL60 myeloid differentiation model into monocytes and granulocytes using vitamin D3 (Vit D3) and all-trans retinoic acid (ATRA) respectively. (B) Bar plot shows mean fluorescence intensity (MFI) of CD14-APC-H7 and CD11b-FITC at 6, 12, 24, 48 and 72 h of induction time points with Vit D3 and ATRA (** = 0.01, *** = 0.001, **** = 0.0001). An unpaired t -test was performed taking a 6 h data point as a reference using GraphPad Prism version 8.2.1. (C) Contour plots showing gating of stained cells with IgG-APC-H7, followed by staining uninduced cells, and Vit D3 induced HL60 cells (72 h induction) with CD14-APC H7 (from left to right contour plots). The X -axis and Y -axis of the contour plots show CD14-APC H7 and forward scatter (A = area) respectively. (D) Bar plots representing MFI of the CD14-APC H7 intensity at 72 h post-Vit D3 induction. The MFI of CD14-APC H7 positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). An unpaired t -test was performed using GraphPad Prism version 8.2.1. (E) Similar to panel C, HL60 cells were induced with ATRA for 72 h and IgG-FITC and CD11b-FITC were used in this experiment. The X -axis and Y -axis of the contour plots show CD11b-FITC and forward scatter (A = area) respectively. (F) Bar plots representing MFI of the CD11b-FITC intensity at 72 h post-ATRA induction. The MFI of CD11b-FITC positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). A statistical test was performed as mentioned in panel D (G) Bar graphs represent the percentage of CD14 positive cells and MFI of CD14-APC H7 positive HL60 cells at 0 and 72 h in RPMI with 10% FBS and IMDM with 20% FBS. The induced cells were compared with their respective uninduced cells in both the culturing conditions in the HL60 differentiation in the IMDM and RPMI media conditions (** = 0.01, *** = 0.001). A statistical test was performed as mentioned in panel (D) (H) Similar to panel G but induction was carried out with ATRA for 72 h. (I) May Grünwald Giemsa staining showing nuclear morphology changes upon HL60 differentiation into monocytes and granulocytes in IMDM (upper panel) and RPMI (lower panel) culturing and induction conditions. The image is taken in Olympus IX83 inverted microscope using CellSens imaging software and 40× magnification.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Molecular and epigenetic alterations in normal and malignant myelopoiesis in human leukemia 60 (HL60) promyelocytic cell line model

doi: 10.3389/fcell.2023.1060537

Figure Lengend Snippet: Differentiation of HL60 model system using two different culturing conditions. (A) Schema of HL60 myeloid differentiation model into monocytes and granulocytes using vitamin D3 (Vit D3) and all-trans retinoic acid (ATRA) respectively. (B) Bar plot shows mean fluorescence intensity (MFI) of CD14-APC-H7 and CD11b-FITC at 6, 12, 24, 48 and 72 h of induction time points with Vit D3 and ATRA (** = 0.01, *** = 0.001, **** = 0.0001). An unpaired t -test was performed taking a 6 h data point as a reference using GraphPad Prism version 8.2.1. (C) Contour plots showing gating of stained cells with IgG-APC-H7, followed by staining uninduced cells, and Vit D3 induced HL60 cells (72 h induction) with CD14-APC H7 (from left to right contour plots). The X -axis and Y -axis of the contour plots show CD14-APC H7 and forward scatter (A = area) respectively. (D) Bar plots representing MFI of the CD14-APC H7 intensity at 72 h post-Vit D3 induction. The MFI of CD14-APC H7 positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). An unpaired t -test was performed using GraphPad Prism version 8.2.1. (E) Similar to panel C, HL60 cells were induced with ATRA for 72 h and IgG-FITC and CD11b-FITC were used in this experiment. The X -axis and Y -axis of the contour plots show CD11b-FITC and forward scatter (A = area) respectively. (F) Bar plots representing MFI of the CD11b-FITC intensity at 72 h post-ATRA induction. The MFI of CD11b-FITC positive cells were compared with unstained cells (UNS), IgG, and uninduced cells (UI) (* = ≤0.05, ** = 0.01). A statistical test was performed as mentioned in panel D (G) Bar graphs represent the percentage of CD14 positive cells and MFI of CD14-APC H7 positive HL60 cells at 0 and 72 h in RPMI with 10% FBS and IMDM with 20% FBS. The induced cells were compared with their respective uninduced cells in both the culturing conditions in the HL60 differentiation in the IMDM and RPMI media conditions (** = 0.01, *** = 0.001). A statistical test was performed as mentioned in panel (D) (H) Similar to panel G but induction was carried out with ATRA for 72 h. (I) May Grünwald Giemsa staining showing nuclear morphology changes upon HL60 differentiation into monocytes and granulocytes in IMDM (upper panel) and RPMI (lower panel) culturing and induction conditions. The image is taken in Olympus IX83 inverted microscope using CellSens imaging software and 40× magnification.

Article Snippet: The American Type Culture Collection (ATCC) recommends the use of IMDM supplemented with 20% FBS for HL60 cell culture ( https://www.atcc.org/products/ccl-240 ), while the European Collection of Authenticated Cell Cultures (ECACC) recommends RPMI 1640 + 2 mM glutamine supplemented with 10% FBS.

Techniques: Fluorescence, Staining, Inverted Microscopy, Imaging, Software